Analysis of SARS-CoV-2 variants B.1.617: host tropism, proteolytic activation, cell-cell fusion, and neutralization sensitivitySARS-CoV-2 변이체 B.1.617 분석: 숙주 친화성, 단백질 분해 활성화, 세포-세포 융합 및 중화 민감도Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, 진단, [키워드] activity Adenovirus adenovirus vaccine Amino acid B.1.617 caused cell-cell fusion cell–cell fusion cells Characteristics Convalescent patients COVID-19 pandemic D614G variant Delta escaped furin host tropism human cells immunized inactivated Inactivated vaccine India Infection Infectivity L452R mAbs Mutation mutations neutralization neutralization activities neutralization activity neutralization. pathogenicity proteolytic activation pseudotyped pseudotyped virus Receptor binding domain reduced SARS-CoV-2 SARS-CoV-2 variant SARS-CoV-2 variants sensitivity sera T478K Transmissibility transmitted variant variants viruses volunteers [DOI] 10.1080/22221751.2022.2054369 PMC 바로가기 [Article Type] Article
SARS-CoV-2 fusion-inhibitory lipopeptides maintain high potency against divergent variants of concern including OmicronSARS-CoV-2 융합 억제 리포펩티드는 오미크론을 포함한 다양한 관심 변종에 대해 높은 효능을 유지합니다Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] activity Alpha antiviral activity Beta broad-spectrum antiviral broad-spectrum antivirals Cell Cell fusion cell-cell fusion combating COVID-19 pandemic D614G Delta drugs effective vaccine Effective vaccines exhibited FIVE fusion inhibitor Gamma Impact Infection inhibiting inhibitor inhibitors Lambda lipopeptide maintain mutant omicron Omicron variant other variant other variants outbreak pseudovirus pseudovirus pseudovirus infection pseudoviruses reduced S protein S proteins SARS-CoV-2 significantly the S protein therapeutic variant variants variants of concern variants of concern. VoC VOCs wild-type WT virus [DOI] 10.1080/22221751.2022.2098060 PMC 바로가기 [Article Type] Article
The Integral Membrane Protein ZMPSTE24 Protects Cells from SARS-CoV-2 Spike-Mediated Pseudovirus Infection and Syncytia FormationArticle Published on 2022-10-262022-11-15 Journal: mBio [Category] COVID19(2023년), SARS, 유전자 메커니즘, 치료제, [키워드] acute respiratory syndrome appear assays catalytic caused Cell cell-cell fusion cell-cell fusion assay cells cellular cellular component cellular membrane contribute coronavirus coronavirus coronavirus SARS-CoV-2 Coronaviruses COVID-19 COVID-19 pandemic dead defend Defense Efficiency ENhance entry of SARS-CoV-2 examined Formation genetic knockout global public health host cell membranes IFITM IFITMs in viral include Infection inhibiting inhibitory activity innate immune innate immune response Integral interact Interaction interfere intrinsic knockdown lentivirus maturation mechanism membrane membrane protein MHV mouse hepatitis virus nuclear overexpression pandemic Plaque assay precursor prelamin A Prevent protease Proteases PROTECT Protective protective role Protein proteolytic proteolytic activity reduce required restriction factor restriction factor. SARS-CoV-2 SARS-CoV-2 pseudovirus infection shown spike Therapeutic benefit transmembrane protein viral infection viral infections virus virus entry viruses wild-type ZMPSTE24 [DOI] 10.1128/mbio.02543-22 PMC 바로가기
Broad Tricyclic Ring Inhibitors Block SARS-CoV-2 Spike Function Required for Viral EntryArticle Published on 2022-10-142022-11-16 Journal: ACS Infectious Diseases [Category] COVID19(2023년), SARS, 변종, [키워드] ACE2 acute respiratory syndrome affected angiotensin-converting enzyme 2 binding binding pocket block blocked broad inhibitor candidate cell-cell fusion cellular circulating SARS-CoV-2 variant Compound conformational change conserved coronavirus Critical Entry inhibitor entry inhibitors entry pathway facilitate function glycoprotein host cell Infection inhibitory effect interfere mode of action plasma membrane receptor Receptor binding domain required Ring SARS-CoV SARS-CoV-1 SARS-CoV-2 SARS-CoV-2 entry SARS-CoV-2 spike selective significantly Small molecules spike Spike. subsequent tested the SARS-CoV-2 these compound Tricyclic Trigger viral entry viral spike [DOI] 10.1021/acsinfecdis.1c00658 PMC 바로가기
Metalloprotease-Dependent S2′-Activation Promotes Cell-Cell Fusion and Syncytiation of SARS-CoV-2Article Published on 2022-09-212022-11-16 Journal: Viruses [Category] SARS, 변종, [키워드] absence ACE2 ACE2 receptor assays benefit cell-cell fusion Combination contribute contributing to COVID-19 ectodomain Factor fusion in vitro induce inhibitor metalloproteases not reduce observation patients with COVID-19 pharmacological promote reduce SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 spike protein SARS-CoV-2 variant serine protease serine proteases significantly Spike protein syncytiation. the SARS-CoV-2 the spike protein treatment strategy [DOI] 10.3390/v14102094 PMC 바로가기
Spike Protein Cleavage-Activation in the Context of the SARS-CoV-2 P681R Mutation: an Analysis from Its First Appearance in Lineage A.23.1 Identified in UgandaArticle Published on 2022-08-312022-11-16 Journal: Microbiology Spectrum [Category] COVID19(2023년), SARS, 변종, [키워드] A.23.1 activated Activation Affect Alpha variant analyzed appearance arginine B.1.1.7 B.1.617 B.1.617.2 cell-cell fusion change changes changes in cleavability cleavage Context Course delta variant functional functional consequences furin furin cleavage site host cell Identified increase in infectivity assay less Lineage mimicking Mutation natural selection notable occur occurred P681R P681R mutation pandemic Particle Pathogenesis peptide performed position 681 predicted proline protease pseudotyped S1/S2 SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 spike significantly increased spike spike gene Spike protein Spike. Spread Surveillance the SARS-CoV-2 Transmissibility transmitted Uganda variant viral variant virus virus [DOI] 10.1128/spectrum.01514-22 PMC 바로가기
Carbohydrate-binding protein from stinging nettle as fusion inhibitor for SARS-CoV-2 variants of concernArticle Published on 2022-08-302022-11-15 Journal: Frontiers in Cellular and Infection Microbiology [Category] SARS, 변종, 치료제, [키워드] addition Antiviral antiviral activity assays Beta binding block Cell cell cultures cell types cell-cell fusion cells circulating Coronaviruses Deletion Delta demonstrated fusion inhibitor Gamma inhibit inhibited inhibitor interface investigated lectin lung epithelial membrane fusion monomeric mutant N-acetylglucosamine (GlcNAc) N-glycosylation omicron Prevent Protein pseudotyped SARS-CoV-2 remained Replication replication of SARS-CoV-2 S2 subunit SARS-CoV-2 SARS-CoV-2 spike SARS-CoV-2 variant Spike protein spike protein. SPR subsequent surface plasmon resonance tested the spike protein UDA Urtica variant variants of concern variants of SARS-CoV-2 Vero E6 cell viral replication viral spike protein virus entry viruses Wuhan-Hu-1 [DOI] 10.3389/fcimb.2022.989534 PMC 바로가기
SARS-CoV-2 spike N-terminal domain modulates TMPRSS2-dependent viral entry and fusogenicitySARS-CoV-2 스파이크 N-말단 도메인은 TMPRSS2 의존성 바이러스 진입 및 융합원성을 조절합니다Article Published on 2022-08-162022-09-11 Journal: Cell Reports [Category] SARS, 변종, [키워드] acute respiratory syndrome acute respiratory syndrome coronavirus acute respiratory syndrome coronavirus 2 airway B.1.617 cell-cell fusion characterized chimeric spike chimeric spikes coronavirus CP: Microbiology Delta enrichment Entry exhibit function Fusogenicity increase Infection Interaction lentivirus Lineage lung cell lung cells Microbiology modulate Mutation N-terminal domain NTD NTDs omicron Organoid organoids pseudotyped Region S1/S2 cleavage S1/S2 cleavage efficiency SARS-CoV-2 SARS-CoV-2 spike severe acute respiratory syndrome Coronavirus spike Spike. TMPRSS2 transfer variants variants of concern viral entry virus entry VOCs wild-type [DOI] 10.1016/j.celrep.2022.111220 PMC 바로가기 [Article Type] Article
Cross-Neutralization of SARS-CoV-2-Specific Antibodies in Convalescent and Immunized Human Sera against the Bat and Pangolin Coronaviruses박쥐 및 천산갑 코로나바이러스에 대한 회복기 및 면역화된 인간 혈청에서 SARS-CoV-2-특이적 항체의 교차 중화Article Published on 2022-08-162022-09-12 Journal: Viruses [Category] SARS, 진단, [키워드] ACE2 analyzed appear bat bat coronavirus binding Cell cell-cell fusion chimeric chimeric proteins contribute coronavirus COVID-19 COVID-19 patient cross-neutralize highest Human human ACE2 receptor human host immunized inactivated induce less neutralization neutralization activity neutralized Neutralizing Neutralizing activity Neutralizing antibodies neutralizing antibody overexpressing pandemic pangolin pangolin coronavirus pangolins pre-existing immunity promote Protein pseudo-virus pseudo-virus. RaTG13 RBD replaced sarbecovirus SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 vaccine serum sample significantly significantly higher spike the RBD the receptor-binding domain thought transmitted vaccination VeroE6 cells while widespread [DOI] 10.3390/v14081793 PMC 바로가기 [Article Type] Article
Novel Engineered SARS-CoV-2 HR1 Trimer Exhibits Improved Potency and Broad-Spectrum Activity against SARS-CoV-2 and Its Variants신규 설계된 SARS-CoV-2 HR1 삼량체, SARS-CoV-2 및 그 변이체에 대해 향상된 효능 및 광범위한 활성을 나타냄Article Published on 2022-07-132022-09-11 Journal: Journal of Virology [Category] COVID19(2023년), SARS, 변종, 치료제, [키워드] activities activity adopted affinity antibodies binding affinity C terminus caused cell-cell fusion develop domain effective Effectiveness foldon foundation fusing fusion fusion inhibitor global public health HR1 HR1 domain Improved increased the risk information inhibit inhibit SARS-CoV-2 inhibitor inhibitors inhibitory inhibitory activity limit MERS MERS-CoV MERS-CoV pseudoviruses motif much higher Multiple NAbs Neutralizing antibodies neutralizing antibody novel pandemic of COVID-19 peptide Peptide inhibitor Peptide Inhibitors peptides potency provide pseudoviruses reported S protein SARS-CoV SARS-CoV-2 SARS-COV-2 infection SARS-CoV-2 variant SARS-CoV-2 variants shown significantly Spread the S protein the SARS-CoV-2 theory treating SARS-CoV-2 infection trimer trimer. trimeric Vaccine variants viral infection viral S protein viral variant Viral variants VOCs [DOI] 10.1128/jvi.00681-22 PMC 바로가기 [Article Type] Article